Action of phenolic antioxidants on various active oxygen species

✍ Scribed by Cynshi, Osamu ;Takashima, Yoshiaki ;Katoh, Yoshiaki ;Tamura, Kunio ;Sato, Mitsuo ;Fujita, Yuzo

• Book ID: 102762121
• Publisher: John Wiley and Sons
• Year: 1995
• Weight: 665 KB
• Volume: 10
• Category: Article
• ISSN: 0884-3996
• DOI: 10.1002/bio.1170100502

✦ Synopsis

The action of phenolic antioxidants, such as probucol, on various active oxygen species was investigated using luminol chemiluminescence and spin trapping w i t h 5,5dimethyl-I -pyrroline-N-oxide (DMPO). The various active oxygen species, including hydroxyl radicals (Fenton reaction), superoxide anions, singlet oxygen and hypochlorite ions were examined w i t h phenolic antioxidants under aqueous and nonaqueous conditions. Probucol showed a quenching effect on both superoxide anions and hypochlorite ions i n nonaqueous solution. However, it had no effect on hydroxyl radicals. a-Tocopherol, a natural phenolic antioxidant, showed a stronger quenching effect on superoxide anions and hypochlorite ions than probucol, and quenched hydroxyl radicals in nonaqueous solution. Furthermore, Trolox showed a quenching effect on all active oxygen species in both aqueous and nonaqueous solution. The antioxidants were studied under comparable conditions in a series of test systems and the reactivity profiles depicted as 'radar charts' which are helpful for characterizing antioxidant action.