𝔖 Bobbio Scriptorium
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Action of mutagenic chemicals on Anacystis nidulans

✍ Scribed by Kumar, H. D.


Publisher
Springer-Verlag
Year
1968
Weight
389 KB
Volume
63
Category
Article
ISSN
0003-9276

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✦ Synopsis


The tolerance of the unicellular blue-green alga Anacystis nidulans to nitrosoguanidine increased from an initial level of 5 ~zg NTG/ml to 20 fzg/ml during the course of 10 successive transfers in NTG-supplemented medium.

Single treatments of log-phase cells with NTG at pH 5--6 resulted in a significant increase in the frequency of streptomycin-resistant mutants but only a marginal increase in the frequency of ultraviolet-resistant mutants was observed following single or 10 successive treatments with NTG.

Attempts to score mutants resistant to penicillin, aminotriazole or NTG were unsuccessful since these agents failed to effectively kill or bleach the sensitive background population on the agar plates.

Following the first reports attributing a weak anti-tumor activity (GOLDIN et al., 1959) and a mutagenie effect (MANDELL and GREENBERG,


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## Abstract Growth of the blue‐green alga __Anacystis nidulans__ treated with methyl methane sulphonate (MMS) at pH 5 or 6 was better than that at pH 7 or 8, both in regard to the duration of the lag‐phase and the growth rate. Toxicity of MMS was directly proportional to the temperature at which th