Action of extremely low frequency electric fields on the cytosolic calcium concentration of differentiated HL-60 cells: Nonactivated cells

✍ Scribed by W. Sontag

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 156 KB
• Volume: 19
• Category: Article
• ISSN: 0197-8462
• DOI: 10.1002/(sici)1521-186x(1998)19:1<32::aid-bem3>3.0.co;2-5

✦ Synopsis

The effect of sinusoidal electric fields on the cytosolic free [Ca2+]i concentration in differentiated HL-60 cells was measured. The calcium concentration was measured in a fluorescence spectrometer using the fluorescence sample fluo-3. In the fluorescence spectrometer two samples can be measured simultaneously, one as the sham-exposed control and the other as the field-exposed sample. The effects of an external field, applied using two capacitor plates outside the cuvettes, and a field applied directly to the medium, using two platinum electrodes inside the cuvettes, were measured at selected frequencies between 0 and 100 Hz and field strengths from 1 to 2000 Vpp/m (external field) and from 0.1 to 1000 Vpp/m (in medium). No significant effects of the fields on the cytosolic free [Ca2+]i concentration in HL-60 cells have been observed at the measured frequencies and field strengths.