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?-actin specific monoclonal antibody

✍ Scribed by Gimona, Mario ;Vandekerckhove, Joel ;Goethals, Marc ;Herzog, Monika ;Lando, Zeev ;Small, J. Victor

Publisher: John Wiley and Sons
Year: 1994
Tongue: English
Weight: 1004 KB
Volume: 27
Category: Article
ISSN: 0886-1544
DOI: 10.1002/cm.970270203

No coin nor oath required. For personal study only.

✦ Synopsis

Using a synthetic peptide mimicking the NH,-terminus of p-actin we have raised a monoclonal antibody specific for this cytoplasmic actin isoform. Specificity of the antibody was demonstrated by its labelling of the actin polypeptide only in tissues containing the p isoform, by its exclusive recognition of the synthetic p-actin peptide amongst those mimicking all six vertebrate isoactins, and by its selective recognition of the p-actin spot in two-dimensional electrophoresis gels of smooth muscle extracts. The antibody bound to actin filaments in both living and fixed fibroblasts where it labelled the stress fiber bundles and, more predominantly, the peripheral actin rich lamellipodia. The characteristics of the antibody indicate that it should serve as a useful tool for studying isoactin distribution and function.

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