Actin isoforms in non-infected roots and symbiotic root nodules ofPhaseolus vulgarisL.
✍ Scribed by Héctor E. Pérez; Nayeli Sánchez; Luis Vidali; J. Manuel Hernández; Miguel Lara; Federico Sánchez
- Book ID
- 104657663
- Publisher
- Springer-Verlag
- Year
- 1994
- Tongue
- English
- Weight
- 683 KB
- Volume
- 193
- Category
- Article
- ISSN
- 0032-0935
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✦ Synopsis
The present report describes an initial characterization of actin from non-infected roots and symbiotic nodules of Phaseolus vulgaris L. cv. Negro damapa. Using anti-actin monoclonal antibodies, a 42-kDa polypeptide was identified in plant extracts. After two-dimensional SDS-PAGE analysis and Western blotting of actin fractions enriched using diethylaminoethyl-resin, the presence of one major isoform of actin in symbiotic nodules and two main isoforms in non-infected roots was revealed. Possible implications of this finding are discussed.