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Acrosome damage of ram spermatozoa during dilution, cooling and freezing

✍ Scribed by Tasseron, F. ;Amir, D. ;Schindler, H.

Book ID
117999707
Publisher
Bioscientifica
Year
1977
Tongue
English
Weight
201 KB
Volume
51
Category
Article
ISSN
1470-1626

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## Abstract Calcium was identified by a pyroantimonate‐osmium fixation technique in ram spermatozoa undergoing a spontaneous acrosome reaction induced by incubation of diluted semen at 39Β°C. Intracellular calcium was only detected in diluted spermatozoa and increased in amount and distribution over