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Acrosomal proteolytic assay for detection of mutagens in mammals

โœ Scribed by L.C. Ginsberg; S.C. Johnson; N. Salama; G. Ficsor

Book ID
113254023
Publisher
Elsevier Science
Year
1981
Weight
318 KB
Volume
91
Category
Article
ISSN
0165-7992

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A rapid, cheap, and sensitive method has been developed for determining proteolytic activity of different classes of endoproteinases. The method is based on a solid-phase assay employing as substrate biotinylated gelatin adsorbed onto microtiter plates. Enzymatic activity is measured by incubating p