Acidic cysteine endoproteinase cathepsin K in the degeneration of the superficial articular hyaline cartilage in osteoarthritis

✍ Scribed by Yrjö T. Konttinen; Jami Mandelin; Tian-Fang Li; Jari Salo; Jan Lassus; Mikko Liljeström; Mika Hukkanen; Michiaki Takagi; Ismo Virtanen; Seppo Santavirta

• Publisher: John Wiley and Sons
• Year: 2002
• Tongue: English
• Weight: 808 KB
• Volume: 46
• Category: Article
• ISSN: 0004-3591
• DOI: 10.1002/art.10185

✦ Synopsis

Abstract

Objective

To measure cartilage pH in patients with osteoarthritis (OA) and to analyze the presence of cathepsin K, the recently discovered acidic endoproteinase, in phenotypically altered chondrocytes.

Methods

Intraoperative measurements of the pH of clinically normal, fibrillated, superficially fissured, and deeply fissured cartilage surfaces (grades 0–3, respectively) in OA patients undergoing primary hip replacement surgery were performed with the use of a sting electrode sterilized with microbicidic plasma. Fluorescent pH probes were used for in situ assessment of cartilage matrix pH. Cathepsin K was assessed using quantitative reverse transcriptase–polymerase chain reaction and immunohistochemistry methods.

Results

The pH of grade 0 cartilage surfaces was 7.1 ± 0.4 (mean ± SD), compared with 6.2 ± 0.9 (P < 0.05), 5.7 ± 1.0 (P < 0.001), and 5.5 ± 1.0 (P < 0.001) for grades 1–3 cartilage surfaces, respectively. Fluorescent pH probes and acid‐dependent autocatalytic conversion of cathepsin K into its active, low molecular weight form in cartilage confirmed these findings. Cathepsin K messenger RNA levels increased in relation to the severity of OA, and the number of cathepsin K–containing chondrocytes increased from a mean ± SD of 12 ± 3 in grade 0 cartilage surfaces to 47 ± 7, 50 ± 6, and 100 ± 12 in grades 1–3 cartilage surfaces, respectively (P < 0.001 for all comparisons).

Conclusion

Acid‐activated, but pharmacologically inhibitable, cathepsin K is induced in phenotypically altered chondrocytes in OA. The findings suggest that cathepsin K, rather than neutral matrix metalloproteinases, degrades the superficial gliding surfaces of the articular hyaline cartilage in OA.