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Acid α-naphthyl acetate esterase assay in murine lymphocytes

✍ Scribed by Janusz Frβckowiak; Andrzej Szutowicz

Book ID
107714448
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
464 KB
Volume
165
Category
Article
ISSN
0003-2697

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✍ Walter Mastropaolo; Joseph Yourno 📂 Article 📅 1981 🏛 Elsevier Science 🌐 English ⚖ 426 KB

A direct, continuous spectrophotometric assay for a-naphthyl acetate esterase and a-naph-thy1 butyrate esterase from human leukocytes has been developed. The method is based on an increase in absorbance at 235 nm which occurs with the hydrolysis of a-naphthyl esters to a-naphthol. This reaction is l