Acid-Stable Amperometric Soybean Peroxidase Biosensor Based on a Self-Gelatinizable Grafting Copolymer of Polyvinyl Alcohol and 4-Vinylpyridine
✍ Scribed by Bingquan Wang; Bin Li; Guangjin Cheng; Shaojun Dong
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 89 KB
- Volume
- 13
- Category
- Article
- ISSN
- 1040-0397
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✦ Synopsis
A peroxidase was extracted from Chinese soybean seed coat, and its thermostability and acid-stability were characterized. This peroxidase was immobilized into a self-gelatinizable grafting copolymer of polyvinyl alcohol with 4-vinylpyridine (PVA-g-PVP) to construct an acidstable hydrogen peroxide biosensor. The effect of pH was studied for optimum analytical performances by amperometric and spectrophotometric methods, also the K m app and the stability of the soybean peroxidase-based biosensor are discussed. At pH 3.0, the soybean peroxidase maintained its bioactivity and the enzyme electrode had a linear range from 0.01 to 6.2 mM with a detection limit of 1.0610 77 M. In addition, the main characteristics of different hydrogen peroxide sensors were compared.