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Acid extraction of tritium label from bacterial DNA in clay sediment

✍ Scribed by M.J. Garet; D.J.W. Moriarty

Book ID
116107401
Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
367 KB
Volume
25
Category
Article
ISSN
0167-7012

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Complete extraction in a form suitable for liquid scintillation counting of tritium-labeled proteins from polyacrylamide gels
✍ Clive Meredith; Martin K. Johnson πŸ“‚ Article πŸ“… 1987 πŸ› Elsevier Science 🌐 English βš– 344 KB

Large (200 mm3) slices of polyacrylamide gels crosslinked with N,N'-diallyltartardiamide which contain tritium-labeled protein are readily solubilized in periodic acid for liquid scintillation counting of radioactivity, but the apparent recovery of label never exceeds 82%. Extraction of the slices w