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Acetylcholinesterase from Schistosoma mansoni: immunological characterization

✍ Scribed by B. Espinoza; R. Tarrab-Hazdai; S. Himmeloch; R. Arnon


Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
856 KB
Volume
28
Category
Article
ISSN
0165-2478

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Acetylcholinesterase of Schistosoma mans
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Larval acetylcholinesterase (acetylcholine acetylhydrolase) EC 3.1.3.7 of the trematode Schistosoma mansoni was characterized and purified by affinity chromatography. The enzyme was solubilized from sonicated cercarial tissue and showed a K, value of 1.83 mM and a V, , , value of 102 U/mg protein. I

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A pool of nuclear proteins from adult worms of Schistosorna rnansoni was analyzed for amino acid cornposition and found to be compatible with high mobility group (HMG) proteins. One of the schistosome HMG proteins was identified as HMG 2 by one-dimensional and two-dimensional PAGE. Stage-specific di