Acetylcholine measurement by high-performance liquid chromatography using an enzyme-loaded postcolumn reactor

✍ Scribed by C. Eva; M. Hadjiconstantinou; N.H. Neff; J.L. Meek

• Book ID: 102629391
• Publisher: Elsevier Science
• Year: 1984
• Tongue: English
• Weight: 403 KB
• Volume: 143
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(84)90670-5

✦ Synopsis

Choline oxidase and cholinesterase were found to retain their activity for 1-2 weeks at room temperature while adsorbed to a commercially available anion-exchange cartridge. These enzymes convert acetylcholine to H2O2. Acetylcholine can be measured in tissue extracts by separation at pH 7 on a polymeric reverse-phase high-performance liquid chromatography column, conversion of acetylcholine to H2O2 on a postcolumn enzyme-loaded anion-exchange cartridge, and electrochemical detection of the H2O2 formed.