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Acetylation of polyamines in mouse brain: Subcellular and regional distribution

✍ Scribed by J. G. Ortiz; E. Giacobini; T. Schmidt-Glenewinkel

Publisher
John Wiley and Sons
Year
1983
Tongue
English
Weight
499 KB
Volume
9
Category
Article
ISSN
0360-4012

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✦ Synopsis

The acetylation of putrescine, cadaverine, spermidine, and spermine was examined in different subcellular fractions and regions of the mouse brain. Acetylation activity was confined to nuclear and microsomal fractions, which can acetylate all of these compounds. These fractions catalyze the formation of N8 but not N'-acetylspermidine. For the nuclear fraction the K, for putrescine was 3.5 mM; for cadaverine, 4.0 mM; for spermidine, 1 .O mM; and for spermine, 2.5 mM. The V,, obtained were (pmol/ mg proteidl0 min): putrescine, 424; cadaverine, 705; spermidine, 239; and spermine,

467.

The acetylation of spermidine was highest in the olfactory bulb and cerebellum. Putrescine and cadaverine acetylation were high in these areas, as well as in the midbrain. Spermine acetylation was rather uniform in all areas examined, except in the brain stem (pons-medulla) where enzyme activity was very low.

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