Acetylation of Cytokinins and Modified Adenine Compounds: A Simple and Non-destructive Derivatization Method for Gas Chromatography–Mass Spectrometric Analysis

✍ Scribed by Per-Olaf Björkman; Elisabeth Tillberg

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 967 KB
• Volume: 7
• Category: Article
• ISSN: 0958-0344
• DOI: 10.1002/(sici)1099-1565(199603)7:2<57::aid-pca283>3.0.co;2-z

✦ Synopsis

Acetic anhydride and N-methylimidazole were used to derivatize more than thirty different cytokinins (bases, nucleosides and nucleotides), N '-modified adenines and adenosines, and the chemically related substances adenosyl-monophosphate, -diphosphate and 4riphosphate. The reaction was allowed to proceed at room temperature for 30 min. There was a high recovery and the derivatives were stable for at least 2 weeks at room temperature when stored in an organic solvent such as toluene, chloroform or acetonitrile. The method was also compared with a commonly used method of acetylation employing acetic anhydride and pyridine as basic catalyst, and with some modifications of it. The electron ionization mass spectra of some acetylated cytokinins are examined, and the fragmentation pathways observed are discussed. Analysis of acetylated bases and nucleosides with gas chromatography-mass spectrometry was possible in the pmol range.