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Acetyl-CoA-Dependent Cleavage of Citrate on Inactivated Citrate Lyase

✍ Scribed by Wolfgang Buckel; Kathrin Ziegert; Hermann Eggerer


Book ID
115113752
Publisher
John Wiley and Sons
Year
1973
Tongue
English
Weight
1001 KB
Volume
37
Category
Article
ISSN
1432-1327

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Methods are described for the assay of ATP citrate (pro-3S)-lyase (EC 4.1.3.8) and acetyl-CoA synthetase (EC 6.2.1.1) activity in crude tissue extracts. Arylamine acetyltransferase (EC 2.3.1.5) and p-nitroaniline serve as indicator system in these tests. The methods have several advantages with resp