Acetaldehyde selectively stimulates collagen production in cultured rat liver fat-storing cells but not in hepatocytes

✍ Scribed by Han Moshage; Alessandro Casini; Charles S. Lieber

• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 925 KB
• Volume: 12
• Category: Article
• ISSN: 0270-9139
• DOI: 10.1002/hep.1840120311

✦ Synopsis

Hepatocytee and fat-storing cells have been implicated in the production of collagen, under both normal and pathological conditions. In thin study, short-term primary cultures of rat hepatocyh-t, maintained in a serum-free, h o r m o d y defined medium without dexamethasone and cultured on a flbronectin-collagem type IV eubetratum, were wed. Primary and p -1 and 2 cultures of fat-storing cells maintained on t h e culture plastic were also studied. Hepatocytes produced significant amounts of collagen type m, but formation of collagen type I was not detectable.

Laminin and collagen type IV production were very low. Hepatocytes maintained their ability to metabolize ethanol (at levels comparable to those observed at 2 hr) for at lea& 48 hr after plating and this metabolism was inhibited 86% to 95% by Pmeth3lpyraaole (1 mmoUL). Neither ethanol (50 mmoUL) nor acetaldehyde (175 pmoUL, initial concentration) had any effect on the production of collagen type m or laminin.

Fat-doring cells (95% to 100% deemin-positive) produced significant amounts of both type I and type III collagen. Production of collagen type IV and laminin was very low. M e t a b o h of ethanol by these cultures was not detected. Addition of ethanol had no effect on collagen or 7 ' ' production in fat-storing cella In contrast, acetaldehyde significantly increased the production of collagen type 1, but did not alter the production of collagen type III, IV or laminin. Incorporation of %-proline into total protein was not affected by addition of ethanol or acetaldehyde to fat-doring cells or hepatocytee. Jkpomwe of fat-storing cells to ethanol or acetaldehyde did not change .Hcollwn degradhg activity in the media. We conclude that fat-storing cells are likely effector cella in the