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Accurate EMCCD Photoelectron Calibration for Single Molecule Imaging Techniques

โœ Scribed by Song, Alexander; Gao, Han; Singh, Avtar; Zipfel, Warren R.

Book ID
122153393
Publisher
Biophysical Society
Year
2013
Tongue
English
Weight
34 KB
Volume
104
Category
Article
ISSN
0006-3495

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## Purpose: To present a practical method for calibrating the zero position of asymmetric jaws that provides higher accuracy at the central axis and improves dose homogeneity in the abutting region of half-beams. ## Methods: Junction doses were measured for each asymmetric jaw using the double-ex