𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Accuracy of herpes simplex virus detection in liquid-based (SurePath) Papanicolaou tests: A comparison with polymerase chain reaction

✍ Scribed by Deniz L. Aslan; Stefan E. Pambuccian; Frances L. Prekker; Timothy W. Schacker; Peter Southern; Kay Savik; Dan McKeon; H. Evin Gulbahce

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
174 KB
Volume
36
Category
Article
ISSN
8755-1039

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

A review of our institution's Papanicolaou test records over an 11‐yr period showed that liquid‐based Papanicolaou tests (LBPTs) had a significantly higher frequency of diagnoses of Herpes simplex virus (HSV)‐related cellular changes compared to conventional Papanicolaou smears (77/302,841, 0.026% vs. 56/376,173, 0.015%, P = 0.002). To investigate the accuracy of the diagnosis of HSV by LBPT, we performed conventional polymerase chain reaction (PCR) on the residual samples from 258 prospectively collected LBPT and real‐time PCR using a different primer set on a subset of 40 LBPT. Conventional PCR was positive in 22 of 22 cases diagnosed of HSV, 1 of 2 cases diagnosed as suspicious for HSV, and none of 234 LBPT without a cytologic HSV diagnosis. Real‐time PCR was positive in 8 of 8 cases diagnosed as HSV and none of the 32 controls. We conclude that LBPT allows an increased detection of HSV that is highly accurate. Diagn. Cytopathol. 2008;36:94–103. © 2008 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Detection of varicella-zoster virus DNA
Detection of varicella-zoster virus DNA in 414 human trigeminal ganglia from cadavers by the polymerase chain reaction: A comparison of the detection rate of varicella-zoster virus and herpes simplex virus type 1
✍ Hiroyuki Inoue; Hisako Motani-Saitoh; Koichi Sakurada; Hiroshi Ikegaya; Daisuke 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 92 KB 👁 1 views

## Abstract Investigation of varicella‐zoster virus (VZV) is important epidemiologically, and determination of its prevalence rate in human trigeminal ganglia is important to provide surveillance data. To date, studies on VZV detection in trigeminal ganglia have used specimens obtained from a relat

Clear detection and typing of herpes sim
Clear detection and typing of herpes simplex virus types 1 and 2 by an indirect ELISA assay: Comparison with three different combined methods—capture ELISA, restriction enzymes, and polymerase chain reaction
✍ Panayotis Markoulatos; Polyxeni Fountoucidou; George Marinakis; Vassilis Krikeli 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 148 KB 👁 2 views

The severity and recurrences of Herpes Simplex Virus (HSV) infection depend on the type of the infectious agent (HSV-1 or HSV-2), which induces the necessity of a nonambiguous detecting typing. The commonly used capture ELISA technique has to be often supported by DNA analysis to confirm the detecti