Accuracy and reliability of flow cytometric DNA analysis using a simple, one-step ethidium bromide staining protocol

One-tube triple staining method for flow

One-tube triple staining method for flow cytometric analysis of DNA ploidy and phenotypic heterogeneity of human solid tumors using single laser excitation

✍ Willem E. Corver; Bert A. Bonsing; Edwin C. A. Abeln; Petra M. Vlak-Theil; Cees 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 920 KB

We have developed a "one-tube" triple staining procedure that allows the identification of intratumor phenotypic subpopulations by FCM. Solid tumors were dissociated by a combined mechanid enzymatic method. Ovarian ascites tumor cell aggregates were enzymatically dissociated using trypsin. A n antik