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Accumulation of insulin-like growth factor binding protein-3 in conditioned medium of human fibroblasts increases with chronologic age of donor and senescence in vitro

โœ Scribed by Samuel Goldstein; Elena J. Moerman; Robert C. Baxter

Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
855 KB
Volume
156
Category
Article
ISSN
0021-9541

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โœฆ Synopsis

We have found that insulin-like growth factor binding protein-3 (IGFBP-3) accumulates to higher levels in medium conditioned by a strain of normal fibroblasts at late passage (LP) and a strain derived from subjects with Werner syndrome (WS) of premature aging, compared to medium conditioned by the same normal cells at early passage (EP) (Goldstein et al., Proc. Natl. Acad. Sci. USA, 1991). To explore the generality of this phenomenon with respect to chronologi- cal age of donor (in vivo aging) and LP (in vitro senescence) we assayed IGFBP-3 in medium conditioned by 18 normal fibroblast strains at EP and LP and two WS strains at the midpoint of their curtailed replicative lifespans and assessed IGFBP-3

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