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Accumulation of fructose 1,6-bisphosphate in mutant cells of mucoidPseudomonas aeruginosaas an evidence of phosphofructokinase activity

✍ Scribed by P. C. Banerjee


Book ID
104762398
Publisher
Springer
Year
1986
Tongue
English
Weight
311 KB
Volume
144
Category
Article
ISSN
0302-8933

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✦ Synopsis


Phosphoglucose isomerase negative mutant of mucoid Pseudomonas aeruginosa accumulated relatively higher concentration of fructose 1,6-bisphosphate (Fru-l,6-Pz) when mannitol induced cells were incubated with this sugar alcohol. Also the toluene-treated cells of fructose 1,6bisphosphate aldolase negative mutant of this organism produced Fru-l,6-P2 from fructose 6-phosphate in presence of ATP, but not from 6-phosphogluconate. The results together suggested the presence of an ATP-dependent fructose 6phosphate kinase (EC 2.7.1.11) in mucoid P. aeruginosa.


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