Accumulation of fructose 1,6-bisphosphate in mutant cells of mucoidPseudomonas aeruginosaas an evidence of phosphofructokinase activity
✍ Scribed by P. C. Banerjee
- Book ID
- 104762398
- Publisher
- Springer
- Year
- 1986
- Tongue
- English
- Weight
- 311 KB
- Volume
- 144
- Category
- Article
- ISSN
- 0302-8933
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✦ Synopsis
Phosphoglucose isomerase negative mutant of mucoid Pseudomonas aeruginosa accumulated relatively higher concentration of fructose 1,6-bisphosphate (Fru-l,6-Pz) when mannitol induced cells were incubated with this sugar alcohol. Also the toluene-treated cells of fructose 1,6bisphosphate aldolase negative mutant of this organism produced Fru-l,6-P2 from fructose 6-phosphate in presence of ATP, but not from 6-phosphogluconate. The results together suggested the presence of an ATP-dependent fructose 6phosphate kinase (EC 2.7.1.11) in mucoid P. aeruginosa.
📜 SIMILAR VOLUMES
## Abstract Treatment of the Class II fructose‐1,6‐bisphosphate aldolase of __Escherichia coli__ with the arginine‐specific α‐dicarbonyl reagents, butanedione or phenylglyoxal, results in inactivation of the enzyme. The enzyme is protected from inactivation by the substrate, fructose 1,6‐bisphospha