𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Accessibility of proteins in 50S ribosomal subunits of Escherichia coli to antibodies: An ultracentrifugation study

✍ Scribed by Morrison, C. A. ;Tischendorf, G. ;St�ffler, G. ;Garrett, R. A.

Publisher
Springer
Year
1977
Tongue
English
Weight
719 KB
Volume
151
Category
Article
ISSN
0026-8925

No coin nor oath required. For personal study only.

✦ Synopsis

The accessibility of each of the proteins on the 50S ribosomal subunit of Escherichia coli was investigated by establishing whether immunoglobulins (IgG), specific for each of the 34 proteins from the 50S subunit, were able to bind to the 50S subunit. The main criterion for accessibility was the formation of specific antibody-50S subunit complexes that could be detected by means of analytical ultracentrifugation. The proteins fell into two main groups. Immunoglobulins against proteins L1, L2, L3, L4, L5, L6, L7/L12, L8, L9, L10, L11, L14, L15, L16, L17, L18, L19, L20, L21, L22, L23, L25, L26, L27 and L30 gave large amounts of complex (20-100%) and, therefore, these proteins were considered to be accessible on the surface of the 50S ribosomal subunit. The antibodies against the remaining proteins L13, L24, L28, L29 and L31 to L34 produced small amounts of complexes (10-20%). Since their effects were unequivocably stronger than those obtained with IgG's from sera of non-immunized animals, the results indicate that these proteins are probably also accessible. Nonetheless, from the ultracentrifugation studies alone definite conclusions about the exposure of the latter group of proteins could not be drawn.

📜 SIMILAR VOLUMES

Prediction for secondary structures of t
Prediction for secondary structures of ten proteins from the 50S subunit of the escherichia coli ribosome
✍ Dzionara, Michael ;Robinson, Stephen Michael Lewis ;Wittmann-Liebold, Brigitte 📂 Article 📅 1977 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 637 KB

## Abstract Predictions of the secondary structures of the following 10 proteins from the large subunit of the E. coli ribosome were made using their known amino acid sequences: L6, L16, L19, L27, L28, L30, L31, L32, L33, and L34. The predictions were made according to 4 different methods and the r

The use of sym-triazine trichloride in R
The use of sym-triazine trichloride in RNA-protein cross-linking studies with Escherichia coli ribosomal subunits
✍ Oste, Christian ;Brimacombe, Richard 📂 Article 📅 1979 🏛 Springer 🌐 English ⚖ 578 KB

The reagent sym-triazine trichloride is used as a bifunctional reagent to generate RNA-protein cross-links within intact ribosomal subunits from E. coli. The reaction takes place in a stepwise manner, involving substitution of one chlorine atom at 12 degrees and pH 8, and substitution of the second

On the GTP-ase activity induced by an ac
On the GTP-ase activity induced by an acidic protein present in 50S ribosomes of Escherichia coli
✍ W. Möller 📂 Article 📅 1972 🏛 Elsevier Science ⚖ 290 KB

Bacterial ribosomes consist of two subunits, the larger 50S and the smaller 30S. Both subunits have a protein to RNA ratio of about 1:2. The larger subunit contains 23S RNA of mol.wt. 1.1 x 106 and the smaller subunit contains 16S RNA of mol.wt. 0.55 X 10 6. The proteins of ribosomes are very comple