ready been praised for its versatility in our previous publications. Advisability of the use of JP thrombin solution in microvascular This time, we compared results of the everting method and the anastomosis was studied as an aid to hemostasis and allow avoidconventional end-to-end method for anastomosing rat femoral ar-ance of the pneumatic tourniquet. teries through periodic observation of anastomosed sites using both the light and scanning electron microscopes. The following results were obtained:
METHODS AND RESULTS
In each anastomosis, damage of intima could be observed, not only at the site of sutures, but also extending into neighboring areas. 2. Regeneration of intima is thought to originate from the damaged intima suture sites. 3. No significant differences between the two methods were observed in regeneration of the intima.
A saline solution of JP thrombin powder (1,ooO U/ml) was compared with saline alone. Adult rats were divided into the following three groups according to method of severing and reanastomosing the femoral artery: (1) the artery was severed in the saline and the intravascular space was washed with the saline before completion Of the suture; (2) the artery was severed in the thrombin solution and was washed with the saline; and (3) the artery was severed in and washed with the thrombin solution.
After 1 week of the surgery, the rates of patency were compared. Whereas 14 rats were used for each group, the rates of patency by groups were (I) 92.9%. (2) 57.1 %, and (3) 35.7%, respectively. Significant differences were noted between groups I and 2 and I and 3, but the difference between groups 2 and 3 was not significant.
CONCLUSION
Use of the thrombin solution significantly lowered the rate of patency of the vessel, which did not improve even by washing with the saline. Thus, the use of thrombin in the field of microvascular Microvascular Anastomosis With Fibrin Glue: Experimental study