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Abstracts 1999 Environmental Mutagen Society Meeting March 27–April 1, 1999 Washington, D.C. James Felton, Chair

Book ID
101265407
Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
214 KB
Volume
33
Category
Article
ISSN
0893-6692

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✦ Synopsis

The impact of air pollution and life style on DNA adducts in placentas was studied in the two areas of the Czech Republic with various annual average air pollution levels of particulate matter <10 µm (PM10) and PAHs; i.e. in the highly polluted area of Northern Bohemia (Teplice District) and in the low polluted agricultural area of Southern Bohemia (Prachatice District). Maternal exposure higher than 40 µg/m 3 PM10 in the first gestational month of pregnancy was found to increase intrauterine growth retardation (IUGR). The placentas' bulky DNA adducts were studied in 279 mothers (191 nonsmokers and 88 smokers). Samples were collected during 1994-95 and 1996-97. DNA adduct levels were determined by butanol extraction enriched procedure of 32 P-postlabeling. The levels of DNA adducts were studied in relation to district, age, weight, height, education of mother, exposure to carcinogenic PAHs in the last month of pregnancy, IUGR, year of sample collection, smoking, metabolic genotypes for GSTM1 and NAT2, vitamin A, E, and C levels in venous and cord blood. Multiple regression analysis revealed that DNA adducts were significantly affected (r=0.746, P<0.001) by smoking (P<0.001), IUGR (P<0.001), NAT2 (P<0.005), exposure to carcinogenic PAHs (P<0.05) and by education of mother (P<0.05). DNA adducts in placentas appear to be sensitive biomarkers of exposure to air pollution and life style. (

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