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The Biochemistry of Vibrio cholerae. I. Growth Methods.--RICHARD \V. LINTON AND ROBERT K. JENNINGS (Archives of Biochemistry, 3: 419, I944). A simple liquid medium capable of producing large crops of cholera vibrios has been developed. K2HPO~, MgSO4, and (NH4)2SO4 supply the mineral requirements, while glucose and a small amount of tryptic digest of casein supply the necessary organic material. Ammonium sulfate also aids in restraining acidification of the culture during growth. Heavy growth is obtained when aeration is carried out vigorously throughout the period Of incubation. The medium may be completely removed from the completed culture by dialysis and evaporation.
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apparatus revealed three components present, whether tlle preparation was derived from H, O, or Vi-containing strains. Each of the three fractions provided some protection against infection with virulent typhoid organisms to mice, but were not in any way superior to the unfractionated concentrate. T
The Biochemistry of Vibrio cholerae. I. Growth Methods.--RICHARD \V. LINTON AND ROBERT K. JENNINGS (Archives of Biochemistry, 3: 419, I944). A simple liquid medium capable of producing large crops of cholera vibrios has been developed. K2HPO~, MgSO4, and (NH4)2SO4 supply the mineral requirements, wh