Abstract of Effect of borate on a protein-polysaccharide complex, the phosphoesterase from calf intestinal mucosa : Charles A. Zittle (Journal of Biological Chemistry, 167: 297. 1947)

• Publisher: Elsevier Science
• Year: 1947
• Tongue: English
• Weight: 66 KB
• Volume: 244
• Category: Article
• ISSN: 0016-0032
• DOI: 10.1016/0016-0032(47)90475-4

✦ Synopsis

in an attempt to fractionate a preparation of phosphodiesterase isolated from calf intestinal mucosa, borate was added prior to ammonium sulfate precipitation.. The solubility of the preparation was reduced by the addition of the borate and a polysaccharide-rich, enzyme-poor fraction could be precipitated by one-half saturation with ammonium sulfate. The enzyme fraction, which could be precipitated at a higher concentration of ammonium sulfate, was twice as active, on a unit weight basis, as the starting material. Phosphodiesterase activity and phosphomonoesterase (alkaline phosphatase) activity were reversibly inhibited approximately 50 per cent. by 0.01 M borate. The data suggest that the enzyme may be a protein-polysaccharide complex.