Absolute quantification of 31P liver metabolites in rat using an external reference and a surface spoiling magnetic field gradient
✍ Scribed by Elke De Bisschop; Robert Luypaert; Guido Annaert; Jean Coremans; Michel Osteaux
- Book ID
- 102960171
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 607 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0952-3480
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✦ Synopsis
Abstract
A simple method for measuring absolute concentrations of ^31^P metabolites in rat liver using an external reference is described. It neutralizes systematic errors due to conductive losses by calibration of the matching capacitor and selects liver signals via an electrically driven, surface spoiling magnetic field gradient. The technique avoids the chemical shift problems associated with linear field gradient localization methods at 4.7 T and allows combinaton with the double standard method for absolute metabolite concentration determination. Application of the method to the in vivo measurement of the absolute concentrations of ATP and P~i~ in eight rat livers yields results that are in good agreement with literature values. Absolute phosphomonoester concentrations were also obtained, but no literature data were available.