Absolute and relative QPCR quantification of plasmid copy number in Escherichia coli
β Scribed by Changsoo Lee; Jaai Kim; Seung Gu Shin; Seokhwan Hwang
- Book ID
- 118471516
- Publisher
- Elsevier Science
- Year
- 2006
- Tongue
- English
- Weight
- 259 KB
- Volume
- 123
- Category
- Article
- ISSN
- 0168-1656
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## Abstract __Escherichia coli__ has been widely used for heterologous protein production (HPP). To determine whether a biofilm environment could benefit __E. coli__ HPP using high copy number plasmids, we compared plasmid maintenance and HPP by __E. coli__ ATCC 33456 containing plasmid pEGFP (a pU