Abnormal intracellular distribution of O6-alkylguanine-DNA-alkyltransferase in hepatitis B cirrhotic human liver: A potential cofactor in the development of hepatocellular carcinoma
✍ Scribed by S M Lee; B C Portmann; G P Margison
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 534 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0270-9139
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✦ Synopsis
Extracts of three of the hepatitis-B liver samples containing 20
The principal carcinogenic and toxic lesion produced in mg of total protein, varying amounts of pure recombinant human ATase protein, and molecular weight markers (Amersham Interna-DNA by N-nitrosocompounds is likely to be O 6 -alkylguanine. tional, Amersham, UK) were subjected to sodium dodecylsulfatepolyacrylamide gel and Western blotting as described previously. 18 Immunostaining procedures using human ATase antiserum or preimmune serum were performed as described previously. [17][18][19] As an addi-Abbreviations: HBV, hepatitis B virus; HCC, hepatocellular carcinoma; ATase, O 6 -alkyltional control, further sections were incubated with ATase antiserum guanine-DNA-alkyltransferase; HBsAg, hepatitis B surface antigen; HBcAg, hepatitis B core antigen. that had been preadsorbed using pure recombinant human ATase.
From the CRC Departments of 1 Carcinogenesis and 2 Medical Oncology, Paterson Insti-Stain development was by a single-step silver intensification of a nickel-