Abnormal hepatic uptake of low doses of sulfobromophthalein in Gilbert's syndrome: The role of reduced affinity of the plasma membrane carrier of organic anions

✍ Scribed by Sandro Gentile; Marcello Persico; Claudio Tiribelli

• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 497 KB
• Volume: 12
• Category: Article
• ISSN: 0270-9139
• DOI: 10.1002/hep.1840120206

✦ Synopsis

The plasma disappearance rate of sulfobromophthalein (VBsp; pmol/kg/min) was measured in 15 Gilbert's syndrome patients and 12 control subjects after intravenous injection of two different doses (0.59 and 6.90 pmol/kg) of the dye. Plasma disappearance rate was significantly reduced in Gilbert's syndrome patients after administration of 0.59 pmol sulfobromophthaleinkg (0.119 f 0.016 vs. 0.146 f 0.018 pmol/kg/min; mean f S.D.; p < O.OOl), whereas no difference was found with the higher dose (0.754 k 0.040 vs. 0.767 +-0.072 pmol/kg/min). Significant reduction was also found after administration to four Gilbert's syndrome patients and four control subjects of 0.29 and 2.96 pmol sulfobromophthalein (0.080 f 0.006 pmol/kg/min vs. 0.077 * 0.07 pmol/kg/min and 0.480 2 0.012 pmol/kg/min vs. 0.691 f 0.016 pmoVkg/min, respectively; p < 0.01). Competition studies with combined administration of sulfobromophthalein (0.59 pmoUkg) and different doses of rifamycin SV (0.69'1.47 and 2.96 pmol/kg) showed a significant (p < 0.001) reduction in plasma disappearance rate in Gilbert's syndrome patients but not in controls. The rifamycin SV dose at which a 50% inhibition in plasma disappearance rate was observed was 0.8 pmol/kg. The apparent affinity (&) of the hepatic trausport was higher in Gilbert's syndrome patients than in control subjects (3.61 f 0.37 pmol sulfobromophthaleidkg vs. 2.76 +-0.29 pmol sulfobromophthaleinkg, mean f S.D.; p < 0.01)' whereas no difference was found in V , , (0.95 +-0.11 pmol sulfobromophthalehkg vs. 0.93 f 0.10 pmol sulfobromophthalein/kg/min, mean f S.D.; N.S.). We conclude that a defective sulfobromo-