Abnormal expression of MUC1 apomucin and mature MUC1 mucin in biliary epithelial cells in various cystic liver diseases
โ Scribed by M Sasaki; Y Nakanuma
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 293 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0270-9139
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โฆ Synopsis
MOTOKO SASAKI AND YASUNI NAKANUMA tigated. [8][9][10][11][12][13][14][15][16][17] However, little is known about the expression of Immunohistochemistry. Sections from each block were immunohistochemically stained for MUC1 apomucin and mature MUC1 mucin, respectively, using the streptavidin-biotin-peroxidase complex (Strept-Abbreviations: BMH, biliary microhamartoma; APCD, adult-type polycystic disease; ABComplex) method. 23 In brief, deparaffinized and rehydrated sec-MHC, multiple hepatic cyst; MAb, monoclonal antibody; IBD, intrahepatic bile duct; SC, tions were immersed in 0.3% hydrogen peroxide (H 2 O 2 ) in methanol solitary cyst. for 20 minutes to abolish endogenous peroxidase activities. The sec-From the
๐ SIMILAR VOLUMES
## Mucins , including MUC-1, are generally considered to be products of epithelial tissues and of their tumors. To examine the possible expression of MUC-1 in other cell types, a panel of human epithelial and non-epithelial tumor cell lines was studied by reverse transcriptase polymerase chain rea
restricted recognition process. (HEPATOLOGY 1996;23: MUC1 apomucin is a specific target tumor antigen rec-1313-1317.) ognized by cytotoxic T cells in a major histocompatibility complex (MHC) unrestricted fashion in patients with pancreatic and breast cancer. This T-cell-mediated im- Mucin is compos