✦ LIBER ✦
Abnormal embryonic development induced by antiserum: A rapid method to isolate the responsible galactoprotein antigens
✍ Scribed by Leung, Christopher C. K.
- Publisher
- John Wiley and Sons
- Year
- 1982
- Tongue
- English
- Weight
- 342 KB
- Volume
- 224
- Category
- Article
- ISSN
- 0022-104X
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✦ Synopsis
Abstract
It has been well established that heterologous antiserum against whole rat kidney homogenate when injected into pregnant rats during the organogenetic period may induce abnormal embryonic development. A one‐step rapid method has been developed to isolate the responsible rat renal antigens by lectin‐affinity chromatography using soy bean agglutinin (SBA) or ricinus com‐munis agglutinin (RCA~120~). The purified antigens appeared to be galactoproteins with terminal nonreducing D‐galactose and/or N‐acetyl‐D‐galactosamine. Rabbit antisera produced against the galactoproteins were potent teratogenic agents.