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Abnormal copper metabolism in menkes cultured fibroblasts

โœ Scribed by T. Onishi; H. Inubushi; S. Tokugawa; M. Muramatsu; K. Nishikawa; Y. Suzuki; M. Miyao

Publisher
Springer
Year
1980
Tongue
English
Weight
524 KB
Volume
134
Category
Article
ISSN
0340-6997

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๐Ÿ“œ SIMILAR VOLUMES

Synthesis of a metallothionein-like prot
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## Abstract A metallothioneinโ€like protein (MTP) is synthesized in normal diploid human skin fibroblasts cultured in Znโ€ or Cuโ€supplemented medium. Synthesis of MTP is not detected in cells cultured without metal supplementation of complete tissueโ€culture medium. Cultured fibroblasts from patients

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## Abstract Proteins of approximately 10,000 daltons (presumably metallothionein) and greater than 75,000 daltons bound ^64^Cu when this metal was added to fibroblast lysates. Treatment with either 2โ€mercaptoethanol or the disodium salt of ethylenediamine tetraacetic acid demonstrated that the high