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Aberrations of the K-ras,p53, andAPC genes in extrahepatic bile duct cancer

✍ Scribed by Suto, Takayuki; Habano, Wataru; Sugai, Tamotsu; Uesugi, Noriyuki; Funato, Osamu; Kanno, Senji; Saito, Kazuyoshi; Nakamura, Shin-ichi

Publisher: John Wiley and Sons
Year: 2000
Tongue: English
Weight: 175 KB
Volume: 73
Category: Article
ISSN: 0022-4790
DOI: 10.1002/(sici)1096-9098(200003)73:3<158::aid-jso9>3.0.co;2-n

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✦ Synopsis

Background and Objectives:

The genetic alterations involved in extrahepatic bile duct (EHBD) cancer are poorly understood. Our aim was to identify aberrations of the K-ras, p53, and APC genes in EHBD cancer. Methods: We investigated aberrations of these genes in 52 EHBD cancers using polymerase chain reaction (PCR) single-strand conformation polymorphism analysis, followed by direct sequence determination and a PCR restriction fragment length polymorphism assay. Results: The K-ras, p53, and APC genes were mutated in 9.6%, 32.7%, and 0% of EHBD cancers, respectively. Loss of heterozygosity at the p53 and APC gene loci was identified in 15.6% and 38.5% of EHBD cancers, respectively. Conclusions: Our results suggest that an unknown suppressor gene on 5q other than the APC gene may be responsible for EHBD cancer.

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