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Aberrant splicing of the type III procollagen mRNA leads to intracellular degradation of the protein in a patient with ehlers-danlos type IV

✍ Scribed by Smita Thakker-Varia; David W. Anderson; Helena Kuivaniemi; Gerard Tromp; Hyeon-Gyu Shin; Michel van der Rest; Francis H. Glorieux; Leena Ala-kokko; Catherine A. Stolle

Publisher: John Wiley and Sons
Year: 1995
Tongue: English
Weight: 965 KB
Volume: 6
Category: Article
ISSN: 1059-7794
DOI: 10.1002/humu.1380060204

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✦ Synopsis

Ehlers-Danlos syndrome type 1V (EDS 1V) is an autosomal dominant disorder characterized by fragile skin, blood vessels, and internal organs and associated with decreased production, secretion, or thermal stability of type 111 procollagen. Mutations in the gene for type 111 procollagen have been identified in patients exhibiting decreased secretion or thermal stability of the protein, but no defect has been elucidated to explain the decreased production of type 111 procollagen in some patients with EDS IV. We report on a patient with a moderate case of EDS IV who produced decreased amounts of type 111 procollagen despite normal levels of translatable type 111 procollagen mRNA. S1 nuclease analysis of the type 111 procollagen mRNA indicated a defect in the region encoding exon 27. Sequence analysis of cDNA clones and genornic fragments generated by polymerase chain reaction amplification revealed that sequences encoded by exon 27 were absent from 3 out of 5 cDNA clones and that a G at the + 5 position of the splice donor site in intron 27 was changed to an A in one allele of the patient's type 111 procollagen gene. Using a cDNA-genomic DNA hybrid probe in S1 nuclease analysis, fragments consistent with mRNA species containing and lacking exon 27 were detected in a 1: 1 ratio. Pulse label and chase experiments in the presence or absence of brefeldin A indicated that most of the type 111 procollagen molecules synthesized by the patient's fibroblasts were not secreted into the medium but were degraded in the endoplasmic reticulum-Golgi compartment by a nonlysosomal mechanism.

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