𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Aberrant methylation of multiple genes in the upper aerodigestive tract epithelium of heavy smokers

✍ Scribed by Sabine Zöchbauer-Müller; Stephen Lam; Shinichi Toyooka; Arvind K. Virmani; Kiyomi O. Toyooka; Sonja Seidl; John D. Minna; Adi F. Gazdar

Publisher
John Wiley and Sons
Year
2003
Tongue
French
Weight
112 KB
Volume
107
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

An important method for silencing tumor suppressor genes in cancers is by aberrant methylation (referred to as methylation) of CpG islands in gene promoter regions. In lung cancer, methylation of the genes retinoic acid receptor beta-2 (RARbeta-2), CDH13 (H-cadherin), p16(INK4a) (p16), RASSF1A (RAS association domain family I) is frequent. Thus, we investigated methylation of these genes in 4 different types of specimens (oropharyngeal brushes, sputum samples, bronchial brushes and bronchioloalveolar lavage [BAL] samples) of the upper aerodigestive tract epithelium from heavy smokers without evidence of cancer but with morphometric evidence of sputum atypia and compared the frequencies of methylation in the different types of specimens. In addition, we also analyzed sputum samples from 30 never smokers for methylation of these genes. Our major findings are: (i) At least one gene was methylated in one or more specimens from 48% of the smokers. However, methylation was statistically significant less frequently in never smokers compared to smokers. (ii) In general, methylation occurred more frequently in samples from the central airways (sputum, bronchial brushes) compared to the peripheral airways (BAL) and only occasionally in the oropharynx. (iii) RARbeta-2 was the most frequently methylated gene, whereas the frequency of methylation for the other genes was lower. (iv) Data from sputum samples and bronchial brushes were comparable. Our findings suggest that detection of methylation should be investigated as an intermediate marker for lung cancer risk assessment and response to chemopreventive regimens.

📜 SIMILAR VOLUMES

Multiple primary tumors of the upper aer
Multiple primary tumors of the upper aerodigestive tract: Is there a role for constitutional mutations in the p53 gene?
✍ Oreste Gallo; Iacopo Sardi; Guglielmina Pepe; Alessandro Franchi; Monica Attanas 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 French ⚖ 229 KB 👁 2 views

Head-and-neck cancer (HNC) patients have a high risk of developing second primary tumors of the upper aerodigestive tract, the main cause of death. Although the roles of tobacco and diet in multiple head-and-neck carcinogenesis have been thoroughly investigated, little is known about individual gene

Family aggregation of carcinoma of the h
Family aggregation of carcinoma of the hypopharynx and cervical esophagus: Special reference to multiplicity of cancer in upper aerodigestive tract
✍ Masaru Morita; Hiroyuki Kuwano; Tadashi Nakashima; Akinobu Taketomi; Hideo Baba; 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 French ⚖ 44 KB 👁 2 views

The role of family history in the multiple occurrence of cancer in the upper aerodigestive tract (UADT) remains unclear. The family histories of close relatives were examined in 167 patients with either hypopharyngeal or cervical esophageal cancer (PhCe cancer) and in 167 control subjects with benig

Multiple occurrence of carcinoma in the
Multiple occurrence of carcinoma in the upper aerodigestive tract associated with esophageal cancer: Reference to smoking, drinking and family history
✍ Masaru Morita; Hiroyuki Kuwano; Shinji Ohno; Keizo Sugimachi; Yosuke Seo; Hirots 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 French ⚖ 439 KB 👁 2 views

## Abstract Smoking and drinking habits as well as family history were examined in 143 men with esophageal cancer, including 30 who had associated second cancers of the upper aerodigestive tract (UADT) and 113 who did not. The risk of second cancers of UADT associated with the main lesions of the e

Smoking and drinking can induce p15 meth
Smoking and drinking can induce p15 methylation in the upper aerodigestive tract of healthy individuals and patients with head and neck squamous cell carcinoma
✍ Hsiao Wen Chang; Guang Sheng Ling; William Ignace Wei; Anthony Po-Wing Yuen 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 209 KB 👁 2 views

## Abstract ## BACKGROUND The consumption of tobacco and alcohol has been implicated in the development of head and neck squamous cell carcinoma (HNSCC). Promoter methylation of tumor suppressor genes is common in HNSCC. In this study, the authors evaluated the effects of tobacco and alcohol on __