A1-mediated adenosine control and ATP P2y site involvement in protein kinase C activity and purine release from cultured astrocytes
β Scribed by CICCARELLI, B; DIIORIO, P; BALLERINI, P; DIMUZIO, M; ALBANESE, C; CACIAGLI, F
- Book ID
- 123345026
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 115 KB
- Volume
- 25
- Category
- Article
- ISSN
- 1043-6618
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ATP-induced arachidonic acid (AA) release was studied in [ 3 H]AAprelabeled cultured astrocytes. To characterize the P 2 purinoceptor-mediated effect of ATP, the subtype-specific agonists 2-methylthio ATP (2-MeSATP) and UTP were compared. ATP, UTP, or 2-MeSATP induced a dose-dependent increase of [
## Abstract A physiological concentration of extracellular ATP stimulated biphasic Ca^2+^ signal, and the Ca^2+^ transient was decreased and the Ca^2+^ sustain was eliminated immediately after removal of ATP and Ca^2+^ in RBAβ2 astrocytes. Reintroduction of Ca^2+^ induced Ca^2+^ sustain. Stimulatio