A well-defined diblock copolymer of poly-(ethylene oxide)-block-poly (4-vinylpyridine) for separation of basic proteins by capillary zone electrophoresis

✍ Scribed by Hang Liu; Ronghua Shi; Wenming Wan; Runmiao Yang; Yanmei Wang

• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 431 KB
• Volume: 29
• Category: Article
• ISSN: 0173-0835
• DOI: 10.1002/elps.200700691

✦ Synopsis

Abstract

A series of well‐defined diblock copolymers, poly(ethylene oxide)‐block‐poly(4‐vinylpyridine) (PEO‐b‐P4VP) used as physical coating of capillaries, were synthesized by atom transfer radical polymerization (ATRP). EOF measurement results showed that all synthesized PEO‐b‐P4VP diblock copolymer‐coated capillaries in this report could suppress EOF effectively compared to the bare fused‐silica capillaries, and efficient separations of basic proteins were achieved. The effects of the molecular weight of P4VP block in PEO‐b‐P4VP and buffer pH on the separation of basic proteins for CE were investigated in detail. Moreover, the relationships between morphologies of PEO‐b‐P4VP diblock copolymers in buffer, which were studied by transmission electron microscopy, and the separation efficiencies of basic protein with PEO‐b‐P4VP diblock copolymers coatings were discussed.