A versatile method to produce antibodies to human T cell receptor Vβ segments: frequency determination of human Vβ2+ T cells that react with toxic-shock syndrome toxin-1

✍ Scribed by François Romagné; Lydia Besnardeau; Bernard Malissen

• Book ID: 102162344
• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 451 KB
• Volume: 22
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830221043

✦ Synopsis

A versatile method to human T cell receptor produce antibodies to Vs segments: frequency determination of human Vp2+ T cells that react with toxic-shock syndrome toxin-l* Human V, (hl$) regions have been expressed in the context of mouse Tcell receptor (TcR)-CD3 complexes, and subsequently used to raise hVg-specific monoclonal antibodies (mAb). The method of expression of hV, outlined in this report contrasts in its versatility with the one reported by Choi et al. (Pvoc. Natl. Acad. Sci. U S A 1991. 88: 8357). For instance, we have applied it successfully to the construction of mouse T cell transfectants expressing hVg1, hVb2, hVp3, hVp8, hVg9, hVp13.5, hVg19, hVp21, and hVp22 gene segments. mAb against the hVp2 and hVp19 regions have been raised by using these transfectants as immunogens in mice. Here, we illustrate the application of the anti-hVg2 mAb to the measurement of human T cells that react with the staphylococcal toxic-shock syndrome toxin-1.