A universal gradient apparatus for multiple organic solvents: II. Fractionation and elution of serum lipids

✍ Scribed by D. Vandamme; B. Declercq; A. Denoo; V. Blaton; H. Peeters

• Publisher: Elsevier Science
• Year: 1975
• Tongue: English
• Weight: 269 KB
• Volume: 69
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(75)90561-8

✦ Synopsis

Chromatography of serum lipids on a silicic acid column by gradient elution and with continuous detection is described. The gradient is formed in a new organic gradient device based on the principle of E. Peterson and H. A. Sober t( 19.59) Anal. Chem. 31, 857), starting from six initial organic solvents. The detection is realized by flame ionization on a LCM? liquid chromatography detector (Pye-Unicam). The technique is less solvent-and time-consuming and is of interest in preparative separation and analysis of lipophilic substances.