A two-step embedding process for better preservation of soft tissue surrounding coral implants
✍ Scribed by Fricain, J. Ch. ;Rouais, F. ;Dupuy, B.
- Book ID
- 102654246
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 622 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0021-9304
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✦ Synopsis
Infiltration of paraffin or embedding polymers proceeds more quickly in soft than in mineralized tissue specimens (bone or biomaterial). The proposed method takes advantage of this difference to protect soft tissue from the action of decalcifying agents. After embedding a bone-softtissue sample in a hydrophobic polymer, it is cleared of the resin on one of its sides to permit access of the decalcifying solution to the mineral component. A second infiltration with another polymerizable solution that cures in the empty pores created by the dissolution makes it possible to obtain a homogeneous hard block, make thin slices, and perform enzyme histochemistry and immunostaining on well-preserved soft tissue with most of its antigenic and enzymatic properties intact. (