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A Two-Component Affinity Chromatography Purification ofHelix pomatiaArylsulfatase by Tyrosine Vanadate

✍ Scribed by Kathryn I. Skorey; Nathalie A. Johnson; Gregory Huyer; Michael J. Gresser


Book ID
115645427
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
153 KB
Volume
15
Category
Article
ISSN
1046-5928

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Adult testicular cytochrome P-450 was purified by a two-step procedure utilizing hydroxylapatite and pregnenolone affinity column chromatography. The cytochrome P-450 was determined to have an isoelectric point of 6.43 on analytical isoelectric focusing. The purified cytochrome P-450 was found to be