A turbidometric assay for measuring proteins in culture media
โ Scribed by Donald Wojciechowicz; Maria De Falco; Ursula Muller-Eberhard
- Book ID
- 118901195
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 336 KB
- Volume
- 106
- Category
- Article
- ISSN
- 0022-1759
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
A semi-automated modification of the protein determination procedure of O. H. Lowry, N. J. Rosebrough, A. L. Farr, and R. J. Randall (1951, J. Biol. Chem. 193, 265-275) is described. The assay is well suited to the analysis of the protein of adherent cultured cells. The procedure is carried out in 9
A macro-dot immunoassay was developed to quantitate proteins (antigens) secreted in the culture media of primary rat hepatocytes. Dilutions of protein standards and undiluted spent culture media were applied to numbered sheets of nitrocellulose (NC) paper by vacuum filtration (in volumes up to I ml)