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A tube-cutting device for fractionating preparative gradients from the ultracentrifuge

✍ Scribed by Roger William Clark; John D. Carnes; Frances E. Arrighi

Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
688 KB
Volume
67
Category
Article
ISSN
0003-2697

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✦ Synopsis

A device is described which cuts off the bottom of a polyallomer (plastic) centrifuge tube, allowing collection of a preparative gradient from the bottom through a large-diameter opening. The gradient flows downward through a funnel-shaped portal positioned below the tube, and the cross-sectional area of flow is gradually restricted. This device resolved into separate peaks DNA sample components differing in density by only 0.007 g/ml in neutral cesium chloride.

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Sucrose and cesium chloride gradient centrifugation are extremely widely used techniques for the fractionation of RNA and ribonucleoprotein particles. A variety of methods have been used to separate high molecular-weight material from gradient fractions as a preliminary to scintillation counting (l-