A Truncated Fibrobacter succinogenes 1,3−1,4-β- d -Glucanase with Improved Enzymatic Activity and Thermotolerance †
✍ Scribed by Wen, Tuan-Nan; Chen, Jui-Lin; Lee, Shu-Hua; Yang, Ning-Sun; Shyur, Lie-Fen
- Book ID
- 127259846
- Publisher
- American Chemical Society
- Year
- 2005
- Tongue
- English
- Weight
- 183 KB
- Volume
- 44
- Category
- Article
- ISSN
- 0006-2960
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## Abstract **BACKGROUND:** 1,3‐1,4‐β‐D‐glucanase (1,3‐1,4‐β‐D‐glucan 4‐glucanohydrolase; EC 3.2.1.73) has been used in a range of industrial processes. As a biocatalyst, it is better to use immobilized enzymes than free enzymes, therefore, the immobilization of 1,3‐1,4‐β‐D‐glucanase was investigat
## Abstract We created 12 mutant enzymes (E11L, F40I, Y42L, N44L, N44Q, E47I, L62G, K64A, K64M, R137M, R137Q, and N139A) from the truncated __Fibrobacter succinogenes__ 1,3–1,4‐β‐D‐glucanase (TF‐glucanase). The enzymes were used to investigate the structural and catalytic roles of specific amino ac