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A tritiated photolabel specific for the active site of monoamine oxidase

✍ Scribed by F. R. C. Backwell; C. H. Williams

Publisher
John Wiley and Sons
Year
1990
Tongue
French
Weight
208 KB
Volume
28
Category
Article
ISSN
0022-2135

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✦ Synopsis

Abstract

We report the synthesis of a tritiated inhibitor of monoamine oxidase, containing a photolabile azido group. The compound, N‐(4‐azido‐2‐nitrophenyl)‐N′‐3‐[^3^H]‐methyl‐N′‐(3‐prop‐1‐ynyl)‐1,3‐diamino propane, is a novel, specific photolabel designed to interact covalently with the flavin of the enzyme in absence of light and to form a second covalent linkage with the polypeptide on photoirradiation. The compound is shown to label a sample of monoamine oxidase isolated from bovine liver, and may be useful in helping to map the active site of the enzyme.

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