𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A transient increase in amino acid transport modulated by insulin in differentiating muscle cells

✍ Scribed by Z. Farfel; S. Karlish; J. Prives

Publisher
John Wiley and Sons
Year
1979
Tongue
English
Weight
313 KB
Volume
98
Category
Article
ISSN
0021-9541

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

During synchronous differentiation of embryonic chick muscle cells in culture, the Na‐dependent uptake of an amino acid analog, α‐amino isobutyric acid (AIB) undergoes an abrupt, transient increase. The increase in AIB uptake is concomitant with the rapid fusion of mononucleated myoblasts, and precedes the accumulation of muscle‐specific proteins. Subsequently, Nadependent AIB transport diminishes markedly during postfusional differentiation of myotubes. The rate of AIB uptake is increased by insulin both before and after myoblast fusion. This stimulation by insulin is restricted to the Nadependent component of total AIB uptake but is apparently not the result of insulin‐mediated increase in the trans‐membrane Na gradient.

📜 SIMILAR VOLUMES

Regulation of amino acid transport and p
Regulation of amino acid transport and protein metabolism in myotubes derived from chicken muscle satellite cells by insulin-like growth factor-I
✍ M. J. Duclos; B. Chevalier; C. Goddard; J. Simon 📂 Article 📅 1993 🏛 John Wiley and Sons 🌐 English ⚖ 896 KB

## Abstract The effects of insulin and insulin‐like growth factor‐I (IGF‐I) on amino acid transport and protein metabolism were compared in myotubes derived from chicken breast muscle satellite cells. Protein synthesis was assessed by continuous labelling with [^3^H]‐tyrosine. Protein degradation w

A reduction in energy-dependent amino ac
A reduction in energy-dependent amino acid transport by microtubular inhibitors in ehrlich ascites tumor cells
✍ Mary Jo Fyfe; Sharon Loftfield; I. David Goldman 📂 Article 📅 1975 🏛 John Wiley and Sons 🌐 English ⚖ 737 KB

## Abstract Vincristine, other periwinkle alkaloids, and colchicine partially inhibit the energy dependent transport of α‐aminoisobutyric acid in Ehrlich ascites tumor cells. The properties of this phenomenon were characterized in detail for vincristine. Maximum depression of the steady‐state intra

Insulin stimulation of glucose transport
Insulin stimulation of glucose transport and metabolism in a human Wilms' tumor-derived myoblast-like cell Line: Modulation of hormone effects by glucose deprivation
✍ Sandra K. Lemmon; Maria G. Buse; Donald A. Sens 📂 Article 📅 1985 🏛 John Wiley and Sons 🌐 English ⚖ 909 KB

The effects of insulin and glucose on parameters of metabolism were investigated in myoblast-like (MBL) cells, a human myoblast-like cell line derived from a Wilms'tumor. Insulin responses were studied after 4 h r pre-incubation in serum free media, with or without 5 m M glucose. Insulin was added d

Membrane transport in synchronized ehrli
Membrane transport in synchronized ehrlich ascites tumor cells: Uptake of amino acids by the A and L system during the cell cycle
✍ Joseph T. Tupper; Barry Mills; Flavia Zorgniotti 📂 Article 📅 1976 🏛 John Wiley and Sons 🌐 English ⚖ 769 KB

Using the double thymidine block technique, Ehrlich ascites tumor cells (ELD) carried i n continuous spinner culture have been synchronized. Simultaneous monitoring of :tH-thymidine incorporation, cell number and mitotic index yielded a cell cycle time of approximately 13.5 hours. This is composed o

System a neutral amino acid transporter
System a neutral amino acid transporter regulation by interleukin-1β in human osteoarthritic synovial cells: evidence for involvement of prostaglandin E2 as a second messenger
✍ Valérie Le Maire; Egle Solito; Françoise Russo-Marie; Alain Hernvann; Hervé Le M 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 275 KB 👁 1 views

We studied the long-terms effects of interleukin-1beta (IL-1beta; 3 to 6 h) on alpha-(methylamino) isobutyric acid (MeAIB), a nonmetabolizable amino acid transported by system A. We found that IL-1beta induced a large decrease in MeAIB uptake by human osteoarthritic synovial cells and a concomitant

Implications of a non-lamellar lipid pha
Implications of a non-lamellar lipid phase for the tight junction stability: Part II: Reversible modulation of transepithelial resistance in high and low resistance MDCK-cells by basic amino acids, Ca2+, protamine and protons
✍ M. Hein; Chr. Madefessel; B. Haag; K. Teichmann; A. Post; H.-J. Galla 📂 Article 📅 1992 🏛 Elsevier Science 🌐 English ⚖ 768 KB

The transepithelial resistance of confluent epithelial cell monolayers was monitored to investigate the influence of basic amino acids, Ca2+, protamine and protons on tight junction electrical resistance. In an accompanying paper we investigated the effect of these substances on the lamellar/hexagon