A microscale method for the isolation of selectively sealed microsomal membrane fractions from plant tissue is presented. The method is based on differential centrifugation in a table top microcentrifuge to accommodate small sample size (10-25 g tissue) and the addition of KI or KCl in the homogeniz
โฆ LIBER โฆ
A TISSUE CULTURE PROCEDURE FOR THE ISOLATION OF CHLAMYDIA PSITTACI FROM KOALAS (PHASCOLARCTOS CINEREUS)
โ Scribed by Grice, RG ;Brown, AS
- Book ID
- 109864572
- Publisher
- Wiley (Blackwell Publishing)
- Year
- 1985
- Weight
- 902 KB
- Volume
- 63
- Category
- Article
- ISSN
- 0004-945X
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Cysteine-proteinases (CP) of the papain family can be affinity-adsorbed by egg white cystatin C coupled to Sepharose 4B, thus allowing their selective isolation from either tissue or cultured cell extracts as well as biolological fluids and culture media. CP complexed by immobilized cystatin are fur