A Three-point Calibration Procedure for Matrix-assisted Laser Desorption/Ionization Mass Spectrometry Utilizing Multiply Charged Ions and Their Mean Initial Velocities
✍ Scribed by César Costa Vera; Roman Zubarev; Hanno Ehring; Per Hakansson; Bo. U. R. Sunqvist
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 404 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0951-4198
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✦ Synopsis
This paper reports the results obtained by using a new calibration procedure for linear time-of-fight matrixassisted laser desorption/ionization mass spectrometry (MALDI). The procedure takes into account the usual MALDI spectra structure by considering the multiply charged molecular ions and the mean initial velocities as a self calibrating property. The mean velocities for all molecular ions are taken to be the same and the procedure is based on the solution of a number of equations (at least three) which relate time-of-flight to mass. Three times better accuracy was obtained in comparison with the normal internal calibration procedure.